polyclonal rabbit anti 17β hsd  (Bioss)

Journal: Scientific Reports

Article Title: Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer ( Cervus elaphus L.)

doi: 10.1038/s41598-021-99601-5

Figure Lengend Snippet: mRNA and protein expression of AKR1C1 ( A , E ), P450 ( B , F ), 3β-HSD ( C , G ) and 17β-HSD ( D , H ) in uterine tissues (endometrium and myometrium) on 4th and 13th day of estrous cycle, in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression and against β-actin (ACTB) for proteins expression. Each bar represents one experimental group with SEM. Statistical differences were analyzed by two-way analysis (ANOVA) of variance followed by the Bonferroni post hoc test using GraphPad PRISM (Version 8.3.0). The lowest statistical significance was P < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* P < 0.1; ** P < 0.01; *** P < 0.001; **** P < 0.0001). Different letters indicate statistical differences ( P < 0.05) between the experimental groups throughout endometrium (A, B) and myometrium (a–b) respectively.

Article Snippet: After that, the membranes was washes three times for 10 min in 1xTBS-T solution and incubated with secondary polyclonal anti-rabbit antibodies for P450, 3β-HSD, 17β-HSD, and AKR1C1 enzymes (Sigma-Aldrich, A3687, 1:10,000), and anti-mouse for PRs and ERα (Sigma-Aldrich, A3562, 1:10,000) for 1.5 h at RT.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer ( Cervus elaphus L.)

doi: 10.1038/s41598-021-99601-5

Figure Lengend Snippet: Oligonucleotide sequences used for Real-time PCR.

Article Snippet: After that, the membranes was washes three times for 10 min in 1xTBS-T solution and incubated with secondary polyclonal anti-rabbit antibodies for P450, 3β-HSD, 17β-HSD, and AKR1C1 enzymes (Sigma-Aldrich, A3687, 1:10,000), and anti-mouse for PRs and ERα (Sigma-Aldrich, A3562, 1:10,000) for 1.5 h at RT.

Techniques: Sequencing, Amplification